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The importance of sample size is greater in quantitative studies compared to qualitative studies. Lack of previous studies in the research area Literature review is an important part of any research, because it helps to identify the scope of works that have been done so far in research area.Literature review findings are used as the foundation for the researcher to be built upon to achieve her research objectives.
While you can use a microscope to view a two-dimensional image of bacteria and record results from that image, you won’t be able to record the height of the bacteria.
The doctors probably tried to make sure that these two groups are similar in demographics, such as having a similar mix of ages, genders and health statuses.
But small differences, such as lifestyle and genetic factors can skew the effects of the drugs on the subjects.
It is for sure that your research will have some limitations and it is normal.
However, it is critically important for you to be striving to minimize the range of scope of limitations throughout the research process.