# Biology Coursework Osmosis - Conclusion

After the tissues have been in the range of sucrose solutions and if necessary they have been stained, I would then observe and estimate the degree of plasmolysis on the cells by viewing them under a microscope.

After the tissues have been in the range of sucrose solutions and if necessary they have been stained, I would then observe and estimate the degree of plasmolysis on the cells by viewing them under a microscope.

To provide more evidence to help make a firm, valid conclusion, a wider range of solutions with reduced intervals in between the sucrose solutions would need to be used.

For example, a range of sucrose solutions of the following concentrations could be prepared: 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 and 1.0 moles/litre. Measuring the change in the diameters of the potato cylinders, as well as the percentage change in mass of the potato cylinders could provide more evidence. I would repeat the experiment more than three times, as this would also provide me with additional evidence to make a firm conclusion. The shape of my graph is similar to my predicted graph and most of the plotted points are close, if not on the best-fit line, indicating accurate and therefore reliable results.

Variables I Would Keep The Same * Length of the Potato Cylinder - If I altered the length of the potato cylinder it would change the surface area of potato exposed to sucrose solutions. I will maintain the length of all the potato cylinders by using a scalpel and ruler to cut and measure lengths.

* Diameter - again, a change would alter the surface area of all the cell membranes exposed to sucrose solutions in turn affecting the rate of osmosis.

Also, I predict that, when the potato cells are placid in a concentration of water that is Isotonic to the cell vacuole contents, there will be no change in mass of the potato cells.

I further predict that unless the plant cell is turgid there will be a percentage change in mass and in the uptake of water into or out of the potato cells wall inversely proportional to the concentration of sucrose.

This means that this is the point where all the water in the cells of the potato have moved out of the cell and there is no solute left to leave. It doesn't pass through the origin, which means that the percentage gain and loss in mass and the concentration are not directly proportional to each other. It shows the percentage gain and loss in mass is inversely proportional to the concentration i.e. It is clear that water will move in and out of the potato cell by osmosis, the aim of the experiment was to determine the solute concentration of the potato cell, which was achieved.

The actual graph show the percentage gain and loss in mass plotted against the molar concentration of sodium chloride (Na Cl). as the concentration increases the percentage gain and loss in mass decreases. **** A sound investigation which could be improved with some simple scientific experimental procedure alterations.

Introduction Aim: To Investigate The Effect Of The Movement Of Water By Osmosis In Plant Cells I will use potato chips as the plant cells in the experiment to show the movement of water by osmosis in and out of plant cells by measuring the mass change, of identical chips, in different concentration solutions of sucrose.

Hypothesis Prediction: I predict that the higher the concentration of water surrounding the potato cells the higher the percentage increase in mass and that the lower the concentration of water surrounding the potato cells the higher the percentage decrease in mass of the potato cells.

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